What is HEPES buffer and its primary application?

HEPES buffer is a zwitterionic organic chemical widely used in biological and biochemical research. Its primary application is to maintain the pH stability of cell culture media and protein extraction buffers. This is essential for experiments requiring precise pH control, especially in environments that are sensitive to fluctuations. HEPES is commonly utilized in areas such as molecular biology, cell culture, and protein biochemistry.

How is the pH of HEPES buffer maintained at 7.3?

The pH of HEPES buffer is typically maintained at 7.3 by adjusting the concentration of HEPES and the ionic strength of the solution. This specific pH is optimal for physiological studies, as it closely mirrors the conditions found in biological systems. When preparing solutions for cell cultures or protein applications, maintaining this pH ensures cellular activities perform optimally, enhancing experimental reliability.

Is this HEPES buffer suitable for cell culture applications?

Yes, this HEPES buffer is specifically designed for cell culture applications. Its buffering capacity at physiological pH levels helps maintain an optimal environment for cells to grow and flourish. You'll find HEPES essential in situations where CO2 levels can cause pH shifts, allowing for a more stable culture environment. This makes it ideal for both routine cell cultivation and specialized experiments.

What are the advantages of using a 0.1um filtration sterilization?

The 0.1um filtration sterilization of HEPES buffer ensures that all potential contaminants such as bacteria and fungi are removed, providing a sterile solution. This is crucial for applications in molecular biology and cell culture, where contamination can compromise experimental results. Using a sterile buffer not only protects your samples but also saves time during the preparation process, allowing you to focus on your research.

Why would I choose a ready-to-use HEPES solution?

A ready-to-use HEPES solution saves you valuable time as it eliminates the need for preparation and pH adjustment. This convenience is particularly beneficial for researchers who require quick access to a reliable buffer during experiments. By reducing preparation times, you can focus on conducting your experiments with greater efficiency and confidence in the consistency of the buffer’s properties.

Can this buffer be used for protein extraction protocols?

Absolutely. This HEPES buffer is suitable for protein extraction protocols as it provides optimal pH and buffering capacity. The stability at pH 7.3 helps to maintain protein structure and activity, minimizing degradation during extraction. It is commonly used for isolating proteins from various cell types, ensuring high yield and functional integrity, crucial for downstream applications such as enzyme assays and mass spectrometry.

What storage conditions are recommended for HEPES buffer?

HEPES buffer should be stored at room temperature in a cool, dry place, away from direct sunlight. This ensures its full efficacy and stability over time. For long-term storage, refrigeration may be beneficial. Keeping the buffer in its original, tightly sealed container helps maintain its pH integrity and prevents contamination, which is essential when performing sensitive experiments.

How do I properly dispose of unused HEPES buffer?

Unused HEPES buffer should be disposed of in accordance with local regulations regarding biological waste. Typically, this involves either neutralizing the solution or following specific disposal procedures outlined by your institution's hazardous waste management protocol. It’s important to not dispose of chemical solutions down the drain without proper treatment, as this can pose environmental hazards.

Er HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, tilgjengelig for nettbutikk i Norway?

Ja, hos Ubuy Norway er dette produktet tilgjengelig for deg å handle til en rimelig pris.. HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, er ikke tilgjengelig lokalt, men du kan stole på oss med våre ekspressfrakttjenester.

Hvor lang tid tar det å få produktet etter bestillingen?

Leveringstiden for det bestilte produktet ditt varierer i henhold til hva du har bestilt og fraktmetoden du har valgt.. Den estimerte leveringstiden er nevnt under utsjekkingsprosessen, så vær bekymringsløs mens du handler.

CryoKing Se original

HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, for Prepare Protein Extraction Buffer, Cell Culture Buffer, Ready to Use Solution, 500ml/Bottle

Ideal for preparing cell culture buffers, protein extraction solutions, and other laboratory applications requiring precise pH control.

CryoKing

vare #: 223448665

HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, for Prepare Protein Extraction Buffer, Cell Culture Buffer, Ready to Use Solution, 500ml/Bottle

Q: Where can I buy HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, for Prepare Protein Extraction Buffer?

You can buy HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, for preparing protein extraction buffer on Ubuy in Norway. Ubuy offers a variety of laboratory-grade products and ensures reliable sourcing for scientific research needs. By choosing Ubuy, you can find your required HEPES buffer quickly and efficiently.

vare #: 223448665

NOK 377

Prisdetaljer

Eksklusive frakt- og tollkostnader ( Frakt- og tollkostnader beregnes ved kassen )

^{*Alle varer importeres fra USA}

Tilgjengelighet : På lager

Importert fra USA-butikk

Antall:

Bestill nå, så får du den omkring Monday, November 02

Sikker transaksjon

Våre beste leveringspartnere

Ideal for preparing cell culture buffers, protein extraction solutions, and other laboratory applications requiring precise pH control.

U-Care-garanti:

Ingen

Velg en plan

Fast
Shipping

Gratis
retur^*

Sikret innpakking

100 % originale produkter

PCI DSS-samsvar

ISO 27001-sertifisert

Note: Step Down Voltage Transformer required for using electronics products of USA store (110-120). Recommended power converters Kjøp nå.

Det som utmerker seg

Sterilization

The HEPES Buffer is filtration sterilized at 0.1um, ensuring the removal of potential contaminants, making it ideal for sensitive applications like protein extraction and cell culture without compromising integrity.

Ready to Use

Pre-mixed at a precise pH of 7.3, this buffer requires no additional preparation, saving time and ensuring consistency in experiments, which is essential for reliable scientific outcomes.

Generous Volume

Supplied in a convenient 500ml bottle, this buffer provides ample quantity for extensive experiments or routine use in laboratory settings, offering great value and reducing the frequency of reorder.

Produkt detaljer

Brand Name	CryoKing
Unit Count	1 Count
Manufacturer	BIOLOGIX
Included Components	HEPES Buffer
Part Number	HEPES-500-1
Brand	CryoKing
Package Weight	1.6 Pound (730 grams)

Hvem passer produktet for?

Cell Biologists
Ideal for researchers needing a stable pH environment for maintaining cell culture viability in experiments.
Protein Researchers
Useful for scientists preparing extraction buffers for proteins, ensuring consistent results in assays and experiments.
Laboratory Technicians
Convenient for technicians requiring ready-to-use solutions for routine cell culture or biochemical assays.

Non-Scientific Users
Not suitable for individuals without lab experience or understanding of buffer solutions and their applications.

PRODUKTBESKRIVELSE

HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, for Prepare Protein Extraction Buffer, Cell Culture Buffer, Ready to Use Solution, 500ml/Bottle

Dietary Supplement Disclaimer

Statements regarding dietary supplements have not been evaluated by the Food and Drug Administration and are not intended to diagnose, treat, cure, or prevent any disease or health condition.

Har du noen spørsmål? Chat med oss

Kundespørsmål og svar

Spørsmål: Hvordan handle HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, online fra Ubuy?

Svar: Det er enkelt å handle HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, online fra Ubuy.. Du trenger bare å søke etter produktet, velge fraktmetode mens du sjekker ut og få det levert til ditt sted.
Spørsmål: Er HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, tilgjengelig for nettbutikk i Norway?

Svar: Ja, hos Ubuy Norway er dette produktet tilgjengelig for deg å handle til en rimelig pris.. HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, er ikke tilgjengelig lokalt, men du kan stole på oss med våre ekspressfrakttjenester.
Spørsmål: Hvor lang tid tar det å få produktet etter bestillingen?

Svar: Leveringstiden for det bestilte produktet ditt varierer i henhold til hva du har bestilt og fraktmetoden du har valgt.. Den estimerte leveringstiden er nevnt under utsjekkingsprosessen, så vær bekymringsløs mens du handler.

CryoKing Buffers Editorial Review

Ingen redaksjonelle anmeldelser funnet

Kundeanmeldelser og vurderinger

5.0

1 kunders vurderinger

5 stjerne

100%
4 stjerne

0%
3 stjerne

0%
2 stjerne

0%
1 stjerne

0%

Vurder dette produktet

Del tankene dine med andre kunder

Fordeler

Ready to use solution
Highly effective for cell culture
consistent pH levels
Sterilized for safety
Convenient 500ml size

Ulemper

Bottle design could be improved.

Produktets prishistorikk

Viktig informasjon

Begrensninger: For produkter som sendes internasjonalt, vær oppmerksom på at eventuell produsentgaranti kan være ugyldig; produsentens serviceordninger er kanskje ikke tilgjengelige; produktmanualer, instruksjoner og sikkerhetsadvarsler er kanskje ikke på mottakerlandets språk; produktene (og medfølgende materiell) er kanskje ikke utformet i samsvar med mottakerlandets standarder, spesifikasjoner og merkekrav; og produktene samsvarer kanskje ikke med mottakerlandets krav til spenning og andre elektriske standarder (noe som kan kreve bruk av adapter eller omformer om nødvendig). Mottakeren er ansvarlig for å sikre at produktet lovlig kan importeres til mottakerlandet. Ved bestilling fra Ubuy eller dets datterselskaper er mottakeren registrert importør og må overholde alle lover og forskrifter i mottakerlandet.
Ikke alle produkter som er oppført på Ubuy er til salgs, da Ubuy er en global søkemotor. Produkter er underlagt eksport-/handelsreguleringer.

NOK 377

Bestill nå, så får du den omkring Monday, November 02

Antall:

Sikker transaksjon

PCI DSS-kompatibel og ISO 27001:2022-sertifisert, med krypterte betalinger og full kjøperbeskyttelse på hver bestilling.

Våre beste leveringspartnere

Raskeste grenseoverskridende levering

Funksjoner og fordeler

Maintains a stable pH of 7.3 ± 0.1 for reliable lab applications.
Low endotoxin levels ensure high purity for sensitive experiments.
Formulated for long-term storage, with a shelf life of at least 3 years.
Batch-to-batch consistency through rigorous testing for quality assurance.
Versatile for various biological and biochemical experiments.
Perfect for cell culture, protein work, and buffer preparations.

Ubuy-garanti

Opplev bekymringsfri shopping med 100 % originale produkter, PCI DSS-kompatibel betalingssikkerhet, ISO 27001-sertifisert databeskyttelse, den raskeste grensekryssende leveringen, gratis retur, og sikker emballasje på hver bestilling.*

HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, for Prepare Protein Extraction Buffer, Cell Culture Buffer, Ready to Use Solution, 500ml/Bottle

NOK 377

Fast Shipping

Gratis retur*

Sikret innpakking

100 % originale produkter

PCI DSS-samsvar

ISO 27001-sertifisert

Det som utmerker seg

Produkt detaljer

Hvem passer produktet for?

PRODUKTBESKRIVELSE

Dietary Supplement Disclaimer

Kundespørsmål og svar

CryoKing Buffers Editorial Review

Kundeanmeldelser og vurderinger

Vurder dette produktet

Fordeler

Ulemper

Produktets prishistorikk

Viktig informasjon

NOK 377

Funksjoner og fordeler

Ubuy-garanti

Fast
Shipping

Gratis
retur^*