What is Proteinase K used for?

Proteinase K is an enzyme primarily used in molecular biology for protein digestion. It effectively breaks down proteins, which helps in purifying nucleic acids by removing contaminating proteins during DNA or RNA extraction processes. In research labs, it is crucial for experiments requiring high-quality nucleic acids, such as PCR or cloning procedures.

How should Proteinase K be stored?

Proteinase K should be stored at -20°C to maintain its stability and activity. Upon reconstitution, it is advisable to aliquot the enzyme into smaller portions to prevent repeated freeze-thaw cycles, which could lead to the loss of enzymatic activity. Proper storage ensures optimal performance in various applications, including DNA and RNA extraction.

What is the optimal activity temperature for Proteinase K?

The optimal temperature for Proteinase K activity is approximately 55-65°C. At this temperature, the enzyme exhibits maximum digestion of proteins, making it highly efficient for protocols requiring thorough protein degradation. Using it at this temperature is essential when processing samples for clean nucleic acid isolation.

Does Proteinase K work with all types of proteins?

Yes, Proteinase K is versatile and can act on a wide range of proteins, including those that are heavily glycosylated or complexed. Its broad specificity makes it invaluable in molecular biology applications, as it can effectively digest various proteins present in cell lysates, thus ensuring the purity of nucleic acids.

Can Proteinase K inactivate viruses?

While Proteinase K is primarily designed for protein digestion, it can also inactivate certain viruses during nucleic acid extraction processes. The enzyme works by degrading viral proteins, thereby assisting in the purification of nucleic acids, essential in virology research where intact nucleic acid is required for further analysis.

What concentration of Proteinase K is typically used in applications?

A typical concentration of Proteinase K used in nucleic acid extraction protocols is between 20 to 100 µg/mL, depending on the sample type and required thoroughness of protein digestion. This range ensures effective removal of contaminants while preserving the quality of the target nucleic acids for downstream applications.

Is Proteinase K suitable for use in diagnostic procedures?

Yes, Proteinase K is suitable for use in certain diagnostic procedures, particularly those involving nucleic acid amplification techniques such as PCR. By ensuring the removal of contaminating proteins, it helps maintain the integrity and reliability of the DNA or RNA being analyzed, which is critical in clinical diagnostics.

What are the safety precautions when handling Proteinase K?

When handling Proteinase K, it is essential to wear appropriate personal protective equipment, including gloves and lab coats, to avoid skin contact. It is also advisable to work in a well-ventilated area to minimize inhalation of any aerosols. Following proper laboratory safety protocols ensures a safe working environment while using the enzyme.

How does Proteinase K compare to other proteases?

Proteinase K has a unique ability to function in a wide pH range and retains activity in the presence of detergents and urea, which sets it apart from other proteases. This property makes it a preferred choice for molecular biology applications over other proteases, providing reliability in results across various applications.

Where can I buy Proteinase K, 100 Milligrams in Norway?

You can purchase Proteinase K, 100 Milligrams from Ubuy in Norway. Ubuy offers a convenient online shopping experience with a range of laboratory products, ensuring you can find the right proteinase for your molecular biology applications delivered right to your door.

Hvordan handle Proteinase K, 100 Milligrams online fra Ubuy?

Det er enkelt å handle Proteinase K, 100 Milligrams online fra Ubuy.. Du trenger bare å søke etter produktet, velge fraktmetode mens du sjekker ut og få det levert til ditt sted.

Er Proteinase K, 100 Milligrams tilgjengelig for nettbutikk i Norway?

Ja, hos Ubuy Norway er dette produktet tilgjengelig for deg å handle til en rimelig pris.. Proteinase K, 100 Milligrams er ikke tilgjengelig lokalt, men du kan stole på oss med våre ekspressfrakttjenester.

Hvor lang tid tar det å få produktet etter bestillingen?

Leveringstiden for det bestilte produktet ditt varierer i henhold til hva du har bestilt og fraktmetoden du har valgt.. Den estimerte leveringstiden er nevnt under utsjekkingsprosessen, så vær bekymringsløs mens du handler.

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Serine protease for protolytic inactivation of nucleases during isolation of DNA and RNA.

RPI

vare #: 11154192

Proteinase K, 100 Milligrams

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vare #: 11154192

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Serine protease for protolytic inactivation of nucleases during isolation of DNA and RNA.

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High Purity

Proteinase K is produced to high purity standards, ensuring reliable performance in enzymatic reactions, which enhances the accuracy of your laboratory results and reduces potential contamination.

Versatile Applications

This enzyme is suitable for a variety of applications, including DNA and RNA extraction, making it an essential tool for molecular biology, diagnostics, and research.

Scalable Quantity

Packaged in 100 milligrams, it provides an ample supply for multiple experiments, allowing laboratories to perform extensive research without frequent reordering, ultimately saving time and costs.

Produkt detaljer

Container Type	Bottle
Item Form	Powder
Item Weight	9.07 g
Brand Name	RPI
Manufacturer	RPI, Corp.
Part Number	P50220-0.1
Brand	RPI
Package Information	Bottle

Hvem passer produktet for?

Molecular Biologists
Ideal for researchers requiring protein degradation in DNA or RNA extraction protocols to enhance yield and purity.
Laboratory Technicians
Useful for technicians involved in clinical applications needing efficient tissue sample processing and protein analysis.
Geneticists
Recommended for genetic studies where enzyme usage is crucial for isolating nucleic acids without contamination.

Home Users
Not suitable for casual users without laboratory experience due to the complexity of properly handling the enzyme.

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Proteinase K, 100 Milligrams

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Spørsmål: Hvordan handle Proteinase K, 100 Milligrams online fra Ubuy?

Svar: Det er enkelt å handle Proteinase K, 100 Milligrams online fra Ubuy.. Du trenger bare å søke etter produktet, velge fraktmetode mens du sjekker ut og få det levert til ditt sted.
Spørsmål: Er Proteinase K, 100 Milligrams tilgjengelig for nettbutikk i Norway?

Svar: Ja, hos Ubuy Norway er dette produktet tilgjengelig for deg å handle til en rimelig pris.. Proteinase K, 100 Milligrams er ikke tilgjengelig lokalt, men du kan stole på oss med våre ekspressfrakttjenester.
Spørsmål: Hvor lang tid tar det å få produktet etter bestillingen?

Svar: Leveringstiden for det bestilte produktet ditt varierer i henhold til hva du har bestilt og fraktmetoden du har valgt.. Den estimerte leveringstiden er nevnt under utsjekkingsprosessen, så vær bekymringsløs mens du handler.

RPI Analytical Reagents Editorial Review

Proteinase K, 100 Milligrams is a high-purity enzyme powder formulated for molecular biology applications. Packaged in a convenient bottle, this product from RPI is designed to facilitate protein digestion and is essential in various laboratory processes. The powdered form allows for easy use and accurate measurement. As a reliable source for molecular research, it has garnered positive feedback for its effectiveness. Users appreciate its consistency and quality, making it a valuable addition to research labs focusing on protein studies or DNA extraction protocols.

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High-purity enzyme for molecular biology applications
Convenient powder form for easy measurement
Reliable source for protein digestion tasks
Positive user feedback on consistency
Effective in DNA extraction protocols

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Might require careful handling in laboratory settings

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“Great products and very good service: very easy and very fast international delivery.”

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“Wonderful online shopping experience, smooth transaction from the start. Payment method works conveniently and delivery is unexpectedly fast and reliable. You go the extra mile for service. What makes this even more amazing, you deliver to Namibia. I will remain a happy Ubuy customer and will increase my purchases for sure! Thank you!”

10 September 2026 · via Trustpilot

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“Very easy to find the products what you need, and so fast delivery, that’s why I highly recommended to others costumers to used ubuy.”

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“I received exactly what I ordered I was skeptical about your site because that was my first time to order. But the order came timely and neatly packaged. I was not disappointed. Thank you.”

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Funksjoner og fordeler

Specifically designed for research or manufacturing use.
Effectively inactivates nucleases, ensuring DNA and RNA integrity.
Essential for accurate isolation processes.
Quality assurance with CAS Number: 39450-01-6.
Not intended for food or drug applications.
Ideal for professionals seeking reliable protolytic solutions.

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Proteinase K, 100 Milligrams

NOK 2164

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Produkt detaljer

Hvem passer produktet for?

PRODUKTBESKRIVELSE

Kundespørsmål og svar

RPI Analytical Reagents Editorial Review

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Fordeler

Ulemper

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NOK 2164

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