What is Tris-HCl Buffer and why is it used?

Tris-HCl Buffer is a widely used buffering agent in biological and chemical applications, primarily due to its ability to maintain a stable pH environment. It is significant in molecular biology applications such as DNA and RNA extraction, electrophoresis, and enzyme assays. By stabilizing the pH, it prevents fluctuations that could compromise experimental results, ensuring consistency and reliability across experiments.

What is the pH level of this Tris-HCl Buffer and its importance?

The Tris-HCl Buffer in question has a pH level of 9.4, which is essential for experiments requiring a slightly basic environment. This pH is particularly beneficial for reactions that involve nucleic acids and certain enzymes that operate optimally at higher pH levels. Maintaining this specific pH helps to improve reaction efficiency and accuracy in various laboratory procedures.

Can I use Tris-HCl Buffer in protein purification?

Yes, Tris-HCl Buffer is commonly employed in protein purification processes. Its capacity to maintain a stable pH is critical during the various stages of protein extraction and purification, minimizing degradation and ensuring protein functionality. The buffer helps maintain solubility and activity of proteins throughout the purification process, making it a staple in biotechnology labs.

How do I prepare Tris-HCl Buffer solutions?

To prepare Tris-HCl Buffer solutions, you can mix Tris base with hydrochloric acid (HCl) at the desired concentration. For a 1M Tris-HCl Buffer at pH 9.4, typically you need to dissolve 121.14 g of Tris base in distilled water, then adjust the pH with concentrated HCl. This precise adjustment ensures that the buffer maintains its target pH for experimental use.

Is Tris-HCl Buffer suitable for cell culture?

Yes, Tris-HCl Buffer can be used in cell culture applications, but it is often combined with other medium components to enhance nutrient availability and maintain pH balance. Its use is prevalent when the exact pH is crucial for cellular activities, such as enzyme functions or metabolic processes, facilitating optimal cell growth.

What are the advantages of using Tris-HCl Buffer over other buffers?

Tris-HCl Buffer offers several advantages, such as a wide buffering range and minimal salt concentration, which is less likely to interfere with biological reactions. Additionally, its relative stability at various temperatures makes it suitable for many laboratory conditions. These properties make Tris-HCl Buffer a preferred choice over other buffering agents in many molecular biology applications.

What applications are best suited for this specific concentration of Tris-HCl Buffer?

The 1M concentration of Tris-HCl Buffer at pH 9.4 is particularly useful in applications involving DNA and RNA manipulation, such as PCR and gel electrophoresis. This concentration helps maintain nucleic acid stability while allowing enzymatic reactions to proceed efficiently, making it essential in both academic and research labs focused on nucleic acid studies.

Can Tris-HCl Buffer be stored, and how long does it last?

Tris-HCl Buffer can be stored at room temperature and should have a relatively long shelf life if kept away from direct sunlight and high temperatures. However, it’s optimal to check for any visible signs of degradation or contamination prior to use. For best results, storing the buffer in tightly sealed containers helps prolong its usability for critical experiments.

Is this buffer compatible with other reagents commonly used in laboratories?

Tris-HCl Buffer is compatible with a variety of common laboratory reagents, including salts, nucleotides, and proteins. However, when using it with sensitive enzymes or compounds, you should verify compatibility, as pH can impact their stability. It’s often mixed with other buffers or reagents to create tailored environments for specific reactions, making it versatile in various protocols.

Where can I buy Tris-HCl Buffer, pH 9.4 [1M] - 100mL?

You can conveniently purchase Tris-HCl Buffer, pH 9.4 [1M] - 100mL from Ubuy, a reliable online marketplace that offers a variety of laboratory supplies. Ubuy provides an efficient shopping experience for scientists and researchers looking for quality reagents and chemicals, ensuring you have access to the necessary tools for your work.

Hvordan handle Tris-HCl Buffer, pH 9.4 [1M] - 100mL online fra Ubuy?

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Er Tris-HCl Buffer, pH 9.4 [1M] - 100mL tilgjengelig for nettbutikk i Norway?

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Tris-HCl Buffer, pH 9.4 [1M] - 100mL

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Optimal pH Stability

This Tris-HCl buffer maintains a precise pH of 9.4, ensuring reliable conditions for biochemical reactions and assays, making it ideal for various laboratory applications.

High Concentration

Being a 1M solution, it provides a robust buffering capacity, effectively stabilizing pH levels even in demanding experimental setups, surpassing lower concentration competitors.

Convenient Packaging

Delivered in a ready-to-use 100mL format, this buffer minimizes preparation time and effort, catering perfectly to researchers seeking efficiency and consistency in their lab work.

Produkt detaljer

Brand Name	eCon Lab Supply
Manufacturer	Ciro Mfg
Included Components	Bottle
Part Number	23.105.53
Item Weight	4 ounces (113.4 grams)
Brand	eCon Lab Supply

Hvem passer produktet for?

Biochemistry Labs
Essential for maintaining stable pH conditions during biochemical assays and experiments in laboratory settings.
Molecular Biology
Ideal for DNA/RNA purification processes where a consistent pH is critical for enzymatic reactions.
Educational Institutions
Useful for teaching purposes in chemistry and biology classes, demonstrating buffer solutions and pH effects.

Acidic Reactions
Not suitable for experiments requiring acidic environments, as Tris-HCl is a basic buffer at pH 9.4.

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Tris-HCl Buffer, pH 9.4 [1M] - 100mL

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Spørsmål: Hvordan handle Tris-HCl Buffer, pH 9.4 [1M] - 100mL online fra Ubuy?

Svar: Det er enkelt å handle Tris-HCl Buffer, pH 9.4 [1M] - 100mL online fra Ubuy.. Du trenger bare å søke etter produktet, velge fraktmetode mens du sjekker ut og få det levert til ditt sted.
Spørsmål: Er Tris-HCl Buffer, pH 9.4 [1M] - 100mL tilgjengelig for nettbutikk i Norway?

Svar: Ja, hos Ubuy Norway er dette produktet tilgjengelig for deg å handle til en rimelig pris.. Tris-HCl Buffer, pH 9.4 [1M] - 100mL er ikke tilgjengelig lokalt, men du kan stole på oss med våre ekspressfrakttjenester.
Spørsmål: Hvor lang tid tar det å få produktet etter bestillingen?

Svar: Leveringstiden for det bestilte produktet ditt varierer i henhold til hva du har bestilt og fraktmetoden du har valgt.. Den estimerte leveringstiden er nevnt under utsjekkingsprosessen, så vær bekymringsløs mens du handler.

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Manufactured in an ISO 9001 and 13485 certified facility in the USA.
Stringent Good Manufacturing Practices followed for quality assurance.
Aseptic filtration ensures product integrity and purity.
Ideal for molecular biology applications, especially nucleic acid purification.
Complete Certificate of Analysis provided for every production lot.
Can be easily diluted to achieve desired concentrations.

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